Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04] (HA721038)
Catalog# HA721038
Rabbit Monoclonal to Oncostatin M
Tested for
-
WB
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IHC-P
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Oncostatin M on different lysates with Rabbit anti-Oncostatin M antibody (HA721038) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721038) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721038) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.