PRODUCT CODE: ET7107-17

OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] (ET7107-17)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.
  • Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.
  • ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] (ET7107-17)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, SiHa, A431, PC-3M, rat epididymis tissue, human liver tissue, human kidney tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB44-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

117 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

OGT

SYNONYMS

FLJ23071 antibody; GlcNAc transferase antibody; HRNT1 antibody; MGC22921 antibody; O GlcNAc antibody; O GlcNAc transferase p110 subunit antibody; O GlcNAc transferase subunit p110 antibody; O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody; O linked N acetylglucosamine (GlcNAc) transferase antibody; O linked N acetylglucosamine transferase 110 kDa subunit antibody; O-GlcNAc transferase subunit p110 antibody; O-linked N-acetylglucosamine transferase 110 kDa subunit antibody; ogt antibody; OGT1_HUMAN antibody; UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody; UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody; Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody

SEQUENCE SIMILARITIES

Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.

TISSUE SPECIFICITY

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its proteasomal degradation.; Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Membrane.

FUNCTION

O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.