Oct4 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A3-C11-R] (HA610085)
Catalog# HA610085
Mouse Monoclonal to Oct4
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA610085) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.