Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Oct-1 Recombinant Rabbit Monoclonal Antibody [JG62-35] (ET7108-30)
Immunogen
Recombinant protein within human oct-1 aa 1-150.
Host
Rabbit
Positive Control
SH-SY-5Y, A431, rat large intestine tissue, human tonsil tissue, human colon cancer tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG62-35
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
Predicted band size 76 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Oct-1
SYNONYMS
FLJ42836 antibody; NF A1 antibody; NF-A1 antibody; Oct 1 antibody; Oct 1B antibody; Oct-1 antibody; OCT1 antibody; Octamer binding protein 1 antibody; Octamer binding transcription factor 1 antibody; Octamer-binding protein 1 antibody; Octamer-binding transcription factor 1 antibody; OTF 1 antibody; OTF-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; OTTHUMP00000032350 antibody; OTTHUMP00000032351 antibody; PO21 antibody; PO2F1 antibody; PO2F1_HUMAN antibody; POU class 2 homeobox 1 antibody; POU domain class 2 transcription factor 1 antibody; POU domain, class 2, transcription factor 1 antibody; POU2F1 antibody
SEQUENCE SIMILARITIES
Belongs to the POU transcription factor family. Class-2 subfamily.
TISSUE SPECIFICITY
Ubiquitous. Isoform 2 is lymphocyte-specific.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene.