PRODUCT CODE: ET7108-30

Oct-1 Recombinant Rabbit Monoclonal Antibody [JG62-35] (ET7108-30)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.
  • Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Oct-1 Recombinant Rabbit Monoclonal Antibody [JG62-35] (ET7108-30)

Immunogen

Recombinant protein within human oct-1 aa 1-150.

Host

Rabbit

Positive Control

SH-SY-5Y, A431, rat large intestine tissue, human tonsil tissue, human colon cancer tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG62-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

Predicted band size 76 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Oct-1

SYNONYMS

FLJ42836 antibody; NF A1 antibody; NF-A1 antibody; Oct 1 antibody; Oct 1B antibody; Oct-1 antibody; OCT1 antibody; Octamer binding protein 1 antibody; Octamer binding transcription factor 1 antibody; Octamer-binding protein 1 antibody; Octamer-binding transcription factor 1 antibody; OTF 1 antibody; OTF-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; OTTHUMP00000032350 antibody; OTTHUMP00000032351 antibody; PO21 antibody; PO2F1 antibody; PO2F1_HUMAN antibody; POU class 2 homeobox 1 antibody; POU domain class 2 transcription factor 1 antibody; POU domain, class 2, transcription factor 1 antibody; POU2F1 antibody

SEQUENCE SIMILARITIES

Belongs to the POU transcription factor family. Class-2 subfamily.

TISSUE SPECIFICITY

Ubiquitous. Isoform 2 is lymphocyte-specific.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene.