PRODUCT CODE: HA500039

OAS3 Rabbit Polyclonal Antibody (HA500039)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: Human placental tissue lysate<br />
Lane 3: A431 cell lysate
  • Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: Human placental tissue lysate<br />
Lane 3: A431 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-OAS3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500039, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Human placental tissue lysate
Lane 3: A431 cell lysate

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

OAS3 Rabbit Polyclonal Antibody (HA500039)

Immunogen

Recombinant protein within human oas3 aa 900-1059.

Host

Rabbit

Positive Control

A549 cell lysate, human placental tissue lysate, A431 cell lysate, human skin tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

121 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC

  • 1:100-1:500

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

OAS3

SYNONYMS

别名; (2-5'')oligo(A) synthase 3 antibody; 2 5' oligo(A) synthetase 3 antibody; 2 5'LIGO antibody; 2 5A synthetase 3 antibody; 2''-5''-oligoadenylate synthase 3 antibody; 2-5A synthase 3 antibody; MGC133260 antibody; OAS3 2' 5' oligoadenylate synthetase 3, 100kDa antibody; OAS3 antibody; OAS3_HUMAN antibody; p100 antibody; p100 OAS antibody; p100OAS antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, Cal-granulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins) and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/ relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in Leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells.