PRODUCT CODE: ET7109-12

NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63] (ET7109-12)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

Western blot analysis of NXF1 on 293 (1) and SiHa (2) cell lysate using anti-NXF1 antibody at 1/2,000 dilution.
  • Western blot analysis of NXF1 on 293 (1) and SiHa (2) cell lysate using anti-NXF1 antibody at 1/2,000 dilution.
  • ICC staining NXF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of NXF1 on 293 (1) and SiHa (2) cell lysate using anti-NXF1 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63] (ET7109-12)

Immunogen

Recombinant protein within c-terminal human nxf1.

Host

Rabbit

Positive Control

293, SiHa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE40-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

NXF1

SYNONYMS

DKFZp667O0311 antibody; DmNXF1 antibody; MEX67 antibody; MEX67, yeast, homolog of antibody; Mex67p antibody; mRNA export factor TAP antibody; Mvb1 antibody; Nuclear RNA export factor 1 antibody; Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody; nxf 1 antibody; NXF1 antibody; NXF1_HUMAN antibody; Protein small bristles antibody; Sbr antibody; TAP antibody; Tip associating protein antibody; Tip-associated protein antibody; Tip-associating protein antibody

SEQUENCE SIMILARITIES

Belongs to the NXF family.

TISSUE SPECIFICITY

Expressed ubiquitously.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (tip-associating protein, also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the nuclear transport factor 2 (NTF2)-like domain.