NusA Mouse Monoclonal Antibody [A6-E7] (M1307-1)
Catalog# M1307-1
Mouse Monoclonal to NusA
Tested for
-
WB
-
ELISA
Reacts with
-
Escherichia coli
Western blot analysis of NusA on E. coli lysates with Mouse anti-NusA antibody (M1307-1) at 1/500 dilution.
Lane 1: E. coli lysate (2 µL/Lane)
Lane 2: E. coli lysate (5 µL/Lane)
Lane 3: E. coli lysate (10 µL/Lane)
Lane 4: E. coli lysate (15 µL/Lane)
Predicted band size: 55 kDa
Observed band size: 65 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1307-1) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: E. coli lysate (2 µL/Lane)
Lane 2: E. coli lysate (5 µL/Lane)
Lane 3: E. coli lysate (10 µL/Lane)
Lane 4: E. coli lysate (15 µL/Lane)
Predicted band size: 55 kDa
Observed band size: 65 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1307-1) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.