PRODUCT CODE: ET1706-25

NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41] (ET1706-25)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

Western blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution.
  • Western blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution.
  • ICC staining NUP153 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NUP153 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NUP153 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41] (ET1706-25)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, HepG2, A431, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU40-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

154 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NUP153

SYNONYMS

153 kDa nucleoporin antibody; HNUP153 antibody; N153 antibody; NU153_HUMAN antibody; Nuclear pore complex protein hnup153 antibody; Nuclear pore complex protein Nup153 antibody; Nucleoporin 153kDa antibody; Nucleoporin Nup153 antibody; Nup 153 antibody; Nup153 antibody

SEQUENCE SIMILARITIES

Belongs to the NUP153 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated in interphase, hyperphosphorylated during mitosis. May play a role in the reversible disassembly of the nuclear pore complex during mitosis (By similarity).; Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.

SUBCELLULAR LOCATION

Nucleus, nuclear pore complex.

FUNCTION

Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup153 is a peripheral NPC component that is implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Nup153 contains a unique N-terminal region, a central domain consisting of four to five zinc fingers and a C-terminal region containing about 30 irregularly spaced FXFG repeats. Nup153 is cleaved by caspases during apoptosis. Nup153 interacts wtih TAP, which is essential for mRNA export, and associates with chromatin towards the end of anaphase, in parallel with the inner nuclear membrane protein LAP2. Nup153 is involved in NPC assembly, in anchoring NPCs within the nuclear envelope and in mediating specific nuclear import events.