NUMB Recombinant Rabbit Monoclonal Antibody [JM10-023] (ET1703-02)
Catalog# ET1703-02
Rabbit Monoclonal to NUMB
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-02) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NUMB Recombinant Rabbit Monoclonal Antibody [JM10-023] (ET1703-02)
Immunogen
Synthetic peptide within human numb aa 4-47 / 651.
Host
Rabbit
Positive Control
A431 cell lysates, Hela, A431, NIH/3T3, mouse brain tissue, rat adrenal gland tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-023
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 71 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NUMB
SYNONYMS
c14_5527 antibody;C14orf41 antibody;FLJ31314 antibody;h Numb antibody;h-Numb antibody;Numb antibody;Numb homolog (Drosophila) antibody;Numb homolog antibody;Numb protein homolog antibody;NUMB_HUMAN antibody;Protein numb homolog antibody;Protein S171 antibody;S171 antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on Ser-276 and Ser-295 by CaMK1.; Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
SUBCELLULAR LOCATION
Endosome membrane, Cell membrane.
FUNCTION
Protein numb homolog is a protein that in humans is encoded by the NUMB gene. The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. The protein Numb is coded for by the gene, NUMB, whose mechanism appears to be evolutionarily conserved. Numb has been extensively studied in both invertebrates and mammals, though its function is best understood in Drosophila. Numb plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. During neurogenesis, Numb localizes to one side of the mother cell such that it is distributed selectively to one daughter cell. This asymmetric division allows a daughter cell containing Numb to acquire a different fate than the other daughter cell.
CITATIONS
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CircCRIM1 suppresses osteosarcoma progression via sponging miR146a-5p and targeting NUMB
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WB
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Human
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Mouse
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Citation
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Zhiqiang He , Yuhan Zhong , Parbatraj Regmi , et al
Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway
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WB
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Human
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Citation
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