PRODUCT CODE: ET7108-55

Nucleostemin Recombinant Rabbit Monoclonal Antibody [JG37-82] (ET7108-55)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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ICC staining Nucleostemin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nucleostemin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nucleostemin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with Nucleostemin antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ICC staining Nucleostemin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Nucleostemin Recombinant Rabbit Monoclonal Antibody [JG37-82] (ET7108-55)

Immunogen

Recombinant protein corresponding to the c-terminus of human nucleostemin.

Host

Rabbit

Positive Control

A549, HepG2, human lung cancer tissue, human breast cancer tissue, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG37-82

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Nucleostemin

SYNONYMS

C77032 antibody; E2 induced gene 3 protein antibody; E2-induced gene 3 protein antibody; E2IG3 antibody; Estradiol induced nucleotide binding protein antibody; GNL3 antibody; GNL3_HUMAN antibody; Guanine nucleotide binding protein like 3 (nucleolar) antibody; Guanine nucleotide binding protein like 3 antibody; Guanine nucleotide-binding protein-like 3 antibody; MGC800 antibody; NNP47 antibody; Novel nucleolar protein 47 antibody; NS antibody; Nucleolar GTP binding protein 3 antibody; Nucleolar GTP-binding protein 3 antibody; Nucleostemin antibody; Putative nucleotide binding protein estradiol induced antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class YlqF/YawG GTPase family.

TISSUE SPECIFICITY

Increased levels in lung tissue in cancer patients.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.