PRODUCT CODE: HA500062

Nucleoside Phosphorylase Rabbit Polyclonal Antibody (HA500062)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of Nucleoside Phosphorylase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500062, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat spleenl tissue lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: K562 cell lysate
  • Western blot analysis of Nucleoside Phosphorylase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500062, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat spleenl tissue lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: K562 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Nucleoside Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500062, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nucleoside Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500062, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Nucleoside Phosphorylase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500062, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat spleenl tissue lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Nucleoside Phosphorylase Rabbit Polyclonal Antibody (HA500062)

Immunogen

Recombinant protein within human nucleoside phosphorylase aa 1-200.

Host

Rabbit

Positive Control

Rat spleenl tissue lysate, Jurkat cell lysate, K562 cell lysate, human placenta tissue, human small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Nucleoside Phosphorylase

SYNONYMS

Purine nucleoside phosphorylase PNP Inosine phosphorylase Inosine-guanosine phosphorylase

SUBCELLULAR LOCATION

Cytoplasm, Cytoskeleton.

FUNCTION

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes inosine into hypoxanthine and guanosine into guanine, in each case creating ribose phosphate. Note: adenosine is first metabolized to inosine via the enzyme adenosine deaminase.One of the reaction catalyzed by purine nucleoside phosphorylase in purine metabolism.Nucleoside phosphorylase is an enzyme which cleaves a nucleoside by phosphorylating the ribose to produce a nucleobase and ribose 1 phosphate. It is one enzyme of the nucleotide salvage pathways. These pathways allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). Often the de novo pathway is interrupted as a result of chemotherapy drugs such as methotrexate or aminopterin.All salvage pathway enzymes require a high energy phosphate donor such as ATP or PRPP.