NSUN2 Recombinant Rabbit Monoclonal Antibody [PSH05-05] (HA722219)
Catalog# HA722219
Rabbit Monoclonal to NSUN2
Tested for
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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IP
Reacts with
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Human
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Mouse
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NSUN2 on different lysates with Rabbit anti-NSUN2 antibody (HA722219) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: 293T cell lysate
Lane 6: A431 cell lysate
Lane 7: HCT 116 cell lysate
Lane 8: Caco-2 cell lysate
Lane 9: Neuro-2a cell lysate
Lane 10: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722219) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: 293T cell lysate
Lane 6: A431 cell lysate
Lane 7: HCT 116 cell lysate
Lane 8: Caco-2 cell lysate
Lane 9: Neuro-2a cell lysate
Lane 10: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722219) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.