NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] (ET1610-96)
Catalog# ET1610-96
Rabbit Monoclonal to NSE
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
-
Zebrafish
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HEK-293 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: SH-SY5Y cell lysate (10 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] (ET1610-96)
Immunogen
Synthetic peptide within human nse aa 30-70.
Host
Rabbit
Positive Control
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, PC-12 cell lysate, SH-SY5Y cell lysate, hybrid fish (crucian-carp) brain tissue lysate, human breast cancer tissue , SH-SY5Y, 293, rat brain tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC06-28
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IF-Cell
-
1:50-1:100
-
IHC-P
-
1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NSE
SYNONYMS
2 phospho D glycerate hydrolyase antibody;2-phospho-D-glycerate hydro-lyase antibody;Eno 2 antibody;ENO2 antibody;ENOG antibody;ENOG_HUMAN antibody;Enolase 2 (gamma, neuronal) antibody;Enolase 2 antibody;Enolase 2 gamma neuronal antibody;Enolase2 antibody;Epididymis secretory protein Li 279 antibody;Gamma enolase antibody;Gamma-enolase antibody;HEL S 279 antibody;Neural enolase antibody;Neuron specific enolase antibody;Neuron specific gamma enolase antibody;Neuron-specific enolase antibody;Neurone specific enolase antibody;NSE antibody
SEQUENCE SIMILARITIES
Belongs to the enolase family.
TISSUE SPECIFICITY
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
DEVELOPMENTAL STAGE
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane.
FUNCTION
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
CITATIONS
-
Cen, S., Liu, K., Zheng, Y., Shan, J., Jing, C., Gao, J., Pan, H., Bai, Z., & Liu, Z.
BRAF Mutation as a Potential Therapeutic Target for Checkpoint Inhibitors: A Comprehensive Analysis of Immune Microenvironment in BRAF Mutated Colon Cancer
-
IHC
-
Human
-
Citation
-
