NSDHL Recombinant Rabbit Monoclonal Antibody [JE64-86] (HA721088)
Catalog# HA721088
Rabbit Monoclonal to NSDHL
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WB
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IF-Cell
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IHC-P
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Human
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NSDHL KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 38 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721088) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NSDHL Recombinant Rabbit Monoclonal Antibody [JE64-86] (HA721088)
Immunogen
Synthetic peptide within human nsdhl aa 151-200/373.
Host
Rabbit
Positive Control
HepG2 cell lysates, Hela cell lysates, human liver tissue, human colon carcinoma tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE64-86
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:200
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IHC-P
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1:400
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NSDHL
SYNONYMS
decarboxylating antibody;H105E3 antibody;H105e3 protein antibody;NAD(P) dependent steroid dehydrogenase like antibody;NSDHL antibody;NSDHL_HUMAN antibody;Protein H105e3 antibody;SDR31E1 antibody;Short chain dehydrogenase/reductase family 31E member 1 antibody;Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating antibody;Sterol-4-alpha-carboxylate 3-dehydrogenase antibody;XAP104 antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Lipid droplet.
FUNCTION
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol. This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis. View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.
CITATIONS
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Yue Liu , Huasong Zhang , Cong Fan , Feiyi Liu , Shaoying Li , Juanjuan Li , Huiying Zhao , Xianhai Zeng
Potential role of Bcl2 in lipid metabolism and synaptic dysfunction of age-related hearing loss
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WB
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Rat
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Citation
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