PRODUCT CODE: ET7109-98

NRG3 Recombinant Rabbit Monoclonal Antibody [JE49-84] (ET7109-98)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

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Western blot analysis of NRG3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human brain tissue lysate<br />
Lane 2: Rat brain tissue lysate
  • Western blot analysis of NRG3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human brain tissue lysate<br />
Lane 2: Rat brain tissue lysate
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-98, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of NRG3 was done on F9 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Western blot analysis of NRG3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: Rat brain tissue lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NRG3 Recombinant Rabbit Monoclonal Antibody [JE49-84] (ET7109-98)

Immunogen

Synthetic peptide within c-terminal human nrg3.

Host

Rabbit

Positive Control

Human brain tissue lysate, rat brain tissue lysate, rat testis tissue, mouse brain tissue, F9.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE49-84

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NRG3

SYNONYMS

HRG3 antibody; Neuregulin 3 antibody; Neuregulin 3 like polypeptide antibody; Neuregulin-3 antibody; Neuregulin3 antibody; NRG-3 antibody; Nrg3 antibody; NRG3_HUMAN antibody; Pro neuregulin 3 antibody; pro NRG3 antibody; Pro-NRG3 antibody

SEQUENCE SIMILARITIES

Belongs to the neuregulin family.

TISSUE SPECIFICITY

Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.

DEVELOPMENTAL STAGE

Isoform 3 is expressed in fetal brain but not in other fetal tissues.

POST-TRANSLATIONAL MODIFICATION

Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.; Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.

SUBCELLULAR LOCATION

Membrane. Secreted.

FUNCTION

Neuregulin 3 also known as NRG3 is a neural-enriched member of the neuregulin protein family which in humans is encoded by the NRG3 gene.[5][6] The NRGs are a group of signaling proteins part of the superfamily of epidermal growth factor; EGF like polypeptide growth factor. These groups of proteins possess an 'EGF-like domain' that consists of six cysteine residues and three disulfide bridges predicted by the consensus sequence of the cysteine residues. Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.