Nrf2 Rabbit Polyclonal Antibody (ER1706-41)
Catalog# ER1706-41
Rabbit Polyclonal to Nrf2
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IHC-P
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IF-Cell
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WB
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Human
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Mouse
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Rat
Immunocytochemistry analysis of HCT 116 cells treated with or without 10 μM MG-132 for 6 hours labeling Nrf2 with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Nrf2 Rabbit Polyclonal Antibody (ER1706-41)
Immunogen
Synthetic peptide within n-terminal human nrf2.
Host
Rabbit
Positive Control
Hela cell lysates, HCT116 cell lysates, HCT 116 cells treated with 10 μM MG-132 for 6 hours, MEF cells treated with 10 μM MG-132 for 6 hours, rat brain tissue, human lung tissue, mouse fallopian tubes tissue, human spleen tissue, human uterus tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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IF-Cell
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1:100
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IHC-P
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1:50-1:200
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WB
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.5% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Nrf2
SYNONYMS
erythroid derived 2 antibody;HEBP1 antibody;like 2 antibody;NF E2 related factor 2 antibody;NF-E2-related factor 2 antibody;NF2L2_HUMAN antibody;NFE2 related factor 2 antibody;NFE2-related factor 2 antibody;Nfe2l2 antibody;Nrf 2 antibody;NRF2 antibody;Nuclear factor (erythroid derived 2) like 2 antibody;Nuclear factor antibody;nuclear factor erythroid 2 like 2 antibody;Nuclear factor erythroid 2 related factor 2 antibody;Nuclear factor erythroid 2-related factor 2 antibody;Nuclear factor erythroid derived 2 like 2 antibody
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1.
CITATIONS
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PD0325901, an ERK inhibitor, attenuates RANKL-induced osteoclast formation and mitigates cartilage inflammation by inhibiting the NF-κB and MAPK pathways
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WB
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Mouse
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Citation
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Wang Ruixuan,et al
Costunolide ameliorates MNNG-induced chronic atrophic gastritis through inhibiting oxidative stress and DNA damage via activation of Nrf2
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IF
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WB
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Human
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Citation
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H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells
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WB
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Mouse
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Citation
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Xin Yang,et al
Cucurbitacin B ameliorates acetaminophen induced mitochondrial oxidative stress through the Nrf2 pathway
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IF
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Citation
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Canonical Wnt signaling works downstream of iron overload to prevent ferroptosis from damaging osteoblast differentiation
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WB
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Mouse
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Citation
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Ze Li,et al
Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway
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WB
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Rat
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Identification, in silico selection, and mechanism study of novel antioxidant peptides derived from the rice bran protein hydrolysates
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WB
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Human
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Citation
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Song Meiying,et al
Gomisin N rescues cognitive impairment of Alzheimer's disease by targeting GSK3β and activating Nrf2 signaling pathway
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WB
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IF
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Mouse
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Citation
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Knockdown of PGC1α suppresses dysplastic oral keratinocytes proliferation through reprogramming energy metabolism
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WB
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Human
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Citation
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Yuwen Li,et al
DDO1002, an NRF2–KEAP1 inhibitor, improves haematopoietic stem cell aging and stress response
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WB
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Mouse
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Citation
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Zixiang Li,et al
Avicularin inhibits ferroptosis and improves cognitive impairments in Alzheimer's disease by modulating the NOX4/Nrf2 axis
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WB
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Mouse
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Citation
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Tang Mi et al
The role of MOTS-c-mediated antioxidant defense in aerobic exercise alleviating diabetic myocardial injury
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WB
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Rat
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Citation
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Protective effect of rutin on ferroptosis‐induced oxidative stress in aging laying hens through Nrf2/HO‐1 signaling
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WB
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Citation
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Wang, L., Zhao, M., Qian, R., Wang, M., Bao, Q., Chen, X., Du, W., Zhang, L., Ye, T., Xie, Y., Zhang, B., Peng, L., & Yao, Y.
Nicotinamide Mononucleotide Ameliorates Silica-Induced Lung Injury through the Nrf2-Regulated Glutathione Metabolism Pathway in Mice
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IHC-P
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WB
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Mouse
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Citation
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Yecheng Wang,et al
Succinate activates UCP2 to suppress neuroinflammation and confer protection following intracerebral hemorrhage
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IHC
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Mouse
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Citation
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Li Yong,et al
A DNA tetrahedron-based ferroptosis-suppressing nanoparticle: superior delivery of curcumin and alleviation of diabetic osteoporosis
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IF
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Mouse
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Citation
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Wu Ziying,et al
Multimodal enhancement of ferroptosis for synergistic cascade colorectal cancer therapy
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WB
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Mouse
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Citation
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Li, H., Liu, N. N., Li, J. R., Dong, B., Wang, M. X., Tan, J. L., Wang, X. K., Jiang, J., Lei, L., Li, H. Y., Sun, H., Jiang, J. D., & Peng, Z. G.
Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease
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WB
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Mouse
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Citation
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He Xinglishang,et al
Dendrobium officinale flowers' topical extracts improve skin oxidative stress and aging
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IHC
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Mouse
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Citation
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