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Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/500 dilution.
Lane 1: Hela cell lysate
Lane 2: HCT116 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
Nrf2 Rabbit Polyclonal Antibody (ER1706-41)
Synthetic peptide within n-terminal human nrf2.
Hela cell lysates, HCT116 cell lysates, Hela, HepG2, Huvec, rat brain tissue, human lung tissue, mouse fallopian tubes tissue, human spleen tissue, human uterus tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1.
Li, H., Liu, N. N., Li, J. R., Dong, B., Wang, M. X., Tan, J. L., Wang, X. K., Jiang, J., Lei, L., Li, H. Y., Sun, H., Jiang, J. D., & Peng, Z. G.
Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease