NR5A2 Recombinant Rabbit Monoclonal Antibody [JE30-50] (HA721379)
Catalog# HA721379
Rabbit Monoclonal to NR5A2
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NR5A2 on different lysates with Rabbit anti-NR5A2 antibody (HA721379) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: A549 cell lysate (15 µg/Lane)
Lane 5: PANC-1 cell lysate (15 µg/Lane)
Lane 6: SW1990 cell lysate (15 µg/Lane)
Lane 7: Mouse liver tissue lysate (30 µg/Lane)
Lane 8: Rat liver tissue lysate (30 µg/Lane)
Lane 9: Mouse brain tissue lysate (30 µg/Lane)
Lane 10: Rat brain tissue lysate (30 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (30 µg/Lane)
Predicted band size: 61 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: A549 cell lysate (15 µg/Lane)
Lane 5: PANC-1 cell lysate (15 µg/Lane)
Lane 6: SW1990 cell lysate (15 µg/Lane)
Lane 7: Mouse liver tissue lysate (30 µg/Lane)
Lane 8: Rat liver tissue lysate (30 µg/Lane)
Lane 9: Mouse brain tissue lysate (30 µg/Lane)
Lane 10: Rat brain tissue lysate (30 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (30 µg/Lane)
Predicted band size: 61 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.