PRODUCT CODE: ER1802-85

NQO1 Rabbit Polyclonal Antibody (ER1802-85)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Siha<br />
   Lane 2: A549
  • Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Siha<br />
   Lane 2: A549
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: A549

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NQO1 Rabbit Polyclonal Antibody (ER1802-85)

Immunogen

Recombinant protein within human nqo1 1-250 aa.

Host

Rabbit

Positive Control

Siha, A549, rat kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NQO1

SYNONYMS

Azoreductase antibody; Cytochrome b 5 reductase antibody; DHQU antibody; DIA 4 antibody; DIA4 antibody; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody; Diaphorase (NADH/NADPH) antibody; Diaphorase 4 antibody; Dioxin inducible 1 antibody; DT diaphorase antibody; DT-diaphorase antibody; DTD antibody; Menadione reductase antibody; NAD(P)H dehydrogenase [quinone] 1 antibody; NAD(P)H dehydrogenase quinone 1 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody; NAD(P)H quinone dehydrogenase 1 antibody; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody; NAD(P)H:menadione oxidoreductase 1 antibody; NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody; NAD(P)H:quinone oxidoreductase 1 antibody; NAD(P)H:quinone oxireductase antibody; NMOR 1 antibody; NMOR I antibody; NMOR1 antibody; NMORI antibody; NQO 1 antibody; NQO1 antibody; NQO1_HUMAN antibody; Phylloquinone reductase antibody; QR 1 antibody; QR1 antibody; Quinone reductase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

CITATIONS

  • Wang, Wanqiu et al.

    Sinomenine attenuates septic-associated lung injury through the Nrf2-Keap1 and autophagy. | The Journal of Pharmacy and Pharmacology [2020]