PRODUCT CODE: EM1801-21

NQO1 Mouse Monoclonal Antibody [13A1] (EM1801-21)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate, untreated <br />
Lane 2: A549 cell lysate, untreated
  • Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate, untreated <br />
Lane 2: A549 cell lysate, untreated
  • Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate, untreated
Lane 2: A549 cell lysate, untreated

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

NQO1 Mouse Monoclonal Antibody [13A1] (EM1801-21)

Immunogen

Recombinant protein within human nqo1 aa 1-285.

Host

Mouse

Positive Control

SiHa, A549, rat uterus tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

13A1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB:1:1,000-1:5,000

  • IHC-P:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NQO1

SYNONYMS

Azoreductase antibody; Cytochrome b 5 reductase antibody; DHQU antibody; DIA 4 antibody; DIA4 antibody; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody; Diaphorase (NADH/NADPH) antibody; Diaphorase 4 antibody; Dioxin inducible 1 antibody; DT diaphorase antibody; DT-diaphorase antibody; DTD antibody; Menadione reductase antibody; NAD(P)H dehydrogenase [quinone] 1 antibody; NAD(P)H dehydrogenase quinone 1 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody; NAD(P)H quinone dehydrogenase 1 antibody; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody; NAD(P)H:menadione oxidoreductase 1 antibody; NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody; NAD(P)H:quinone oxidoreductase 1 antibody; NAD(P)H:quinone oxireductase antibody; NMOR 1 antibody; NMOR I antibody; NMOR1 antibody; NMORI antibody; NQO 1 antibody; NQO1 antibody; NQO1_HUMAN antibody; Phylloquinone reductase antibody; QR 1 antibody; QR1 antibody; Quinone reductase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.