PRODUCT CODE: ER1901-14

NPY2R / Y2 receptor Rabbit Polyclonal Antibody (ER1901-14)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of NPY2R / Y2 receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NPY2R / Y2 receptor Rabbit Polyclonal Antibody (ER1901-14)

Immunogen

Synthetic peptide within mouse npy2r / y2 receptor aa 327-376 / 381.

Host

Rabbit

Positive Control

MCF-7 cell lysates.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

43 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NPY2R / Y2 receptor

SYNONYMS

Neuropeptide Y receptor type 2 antibody; Neuropeptide Y receptor Y2 antibody; NPY-Y2 receptor antibody; NPY2-R antibody; NPY2R antibody; NPY2R_HUMAN antibody; Y2 receptor antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Neuropeptide Y (NPY) and its family of five receptors are known to be associated with hypothalamic regulation of feeding behavior, metabolism, and energy homeostasis in both rodents and humans. In particular, knock-out mice for the gene neuropeptide Y 2 receptor, NPY2R, exhibit hyperphagia and excessive weight gain. As a result, research on the gene has focused on genotyping analysis for the identification of single nucleotide polymorphisms (SNPs) in obese human subjects and drug development for manipulation of its expression as a novel therapeutic treatment for obesity. Although primarily expressed in the arcuate nucleus of the hypothalamus, NPY2R may also be found in other tissues including trabecular bone, vascular tissue, and colonic mucosa.