PRODUCT CODE: ET7108-72

NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31] (ET7108-72)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NOXA2 on rat spleen tissue lysate using anti-NOXA2 antibody at 1/500 dilution.
  • Western blot analysis of NOXA2 on rat spleen tissue lysate using anti-NOXA2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.
Western blot analysis of NOXA2 on rat spleen tissue lysate using anti-NOXA2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31] (ET7108-72)

Immunogen

Recombinant protein within human noxa2 aa 150-300.

Host

Rabbit

Positive Control

Rat spleen tissue, human spleen tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG86-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60/47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

NOXA2

SYNONYMS

67 kDa neutrophil oxidase factor antibody; Chronic granulomatous disease autosomal 2 antibody; FLJ93058 antibody; NADPH oxidase activator 2 antibody; NCF-2 antibody; Ncf2 antibody; NCF2_HUMAN antibody; Neutrophil cytosol factor 2 antibody; Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody; Neutrophil NADPH oxidase factor 2 antibody; NOXA2 antibody; P67 PHOX antibody; p67-phox antibody; p67phox antibody

SEQUENCE SIMILARITIES

Belongs to the NCF2/NOXA1 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67phox. The cytosolic proteins p47phox and p67phox, also designated neutrophil cytosol factor 1(NCF1) and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67phox has been shown to interact with the proline rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67phox to the membrane.