PRODUCT CODE: ET1703-20

NOTCH4 Recombinant Rabbit Monoclonal Antibody [JM101-05] (ET1703-20)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

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Flow cytometric analysis of Hela cells with NOTCH4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Flow cytometric analysis of Hela cells with NOTCH4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Flow cytometric analysis of Hela cells with NOTCH4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NOTCH4 Recombinant Rabbit Monoclonal Antibody [JM101-05] (ET1703-20)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, human kdiney tissue, mouse lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM101-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -81C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

210 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NOTCH4

SYNONYMS

FLJ16302 antibody; hNotch 4 antibody; hNotch4 antibody; INT3 antibody; Neurogenic locus notch homolog protein 4 antibody; NOTC4_HUMAN antibody; Notch 4 antibody; Notch 4 intracellular domain antibody; Notch homolog 4 antibody; NOTCH3 antibody; Notch4 antibody; NOTCH4 protein antibody

SEQUENCE SIMILARITIES

Belongs to the NOTCH family.

TISSUE SPECIFICITY

Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

POST-TRANSLATIONAL MODIFICATION

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).; Phosphorylated.

SUBCELLULAR LOCATION

Cell membrane, Nucleus.

FUNCTION

The LIN-12/Notch transmembrane receptors are believed to play a central role in development by regulating cell fate decisions. Four Notch homologs (Notch 1, Notch 2, Notch 3 and Notch 4) have been identified in mammals. The Notch genes are expressed in a variety of embryonic and adult tissues, suggesting that the genes are involved in multiple signaling pathways. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged1 can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 may be involved in tissue development that is dependent upon epithelial-mesenchymal interactions. In addition to its normal expression in the adrenal gland and placenta, Delta expression has also been found in neuroendocrine tumors.