non-muscle Myosin IIA Rabbit Polyclonal Antibody (ER62480)
Catalog# ER62480
Rabbit Polyclonal to non-muscle Myosin IIA
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of non-muscle Myosin IIA on different lysates with Rabbit anti-non-muscle Myosin IIA antibody (ER62480) at 1/10,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Mouse liver tissue lysate (40 µg/Lane)
Lane 9: Rat kidney tissue lysate (40 µg/Lane)
Lane 10: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 227 kDa
Observed band size: 227 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62480) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Mouse liver tissue lysate (40 µg/Lane)
Lane 9: Rat kidney tissue lysate (40 µg/Lane)
Lane 10: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 227 kDa
Observed band size: 227 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62480) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.