PRODUCT CODE: ER1802-30

Noggin Rabbit Polyclonal Antibody (ER1802-30)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.
  • Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.
  • ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Noggin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with Noggin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Noggin Rabbit Polyclonal Antibody (ER1802-30)

Immunogen

Recombinant full length protein of human noggin.

Host

Rabbit

Positive Control

Noggin Recombinant protein, PC-3M, rat liver tissue, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Noggin

SYNONYMS

Nog antibody; NOGG_HUMAN antibody; Noggin antibody; SYM 1 antibody; SYM1 antibody; Symphalangism 1 (proximal) antibody; Synostoses (multiple) syndrome 1 antibody; SYNS 1 antibody; SYNS1 antibody

SEQUENCE SIMILARITIES

Belongs to the noggin family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Genetic differentiation of the vertebrate somite necessitates a balance of inductive signals and antagonists. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGFβ) superfamily of signaling proteins, such as bone morphogenetic proteins-2, 4, 7 (BMP2, BMP4, BMP7). Inhibition of BMP signaling by axially secreted Noggin mediates normal vertebrate skeletogenesis and patterning of the neural tube and somite. Spatially, Noggin may effectively antagonize BMP activity by efficiently diffusing through extracellular matrices, thereby creating morpho-genic gradients. Mice embryos that are homozygous null for Noggin, a lethal genotype, display stubby, continuous limbs with lack of joints in the paws and an array of other developmental defects.