PRODUCT CODE: 0805-2

Noggin Rabbit Polyclonal Antibody (0805-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Noggin on MG-63 cell lysate using anti-Noggin antibody at 1/1,000 dilution.
  • Western blot analysis of Noggin on MG-63 cell lysate using anti-Noggin antibody at 1/1,000 dilution.
  • ICC staining Noggin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Noggin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
Western blot analysis of Noggin on MG-63 cell lysate using anti-Noggin antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Noggin Rabbit Polyclonal Antibody (0805-2)

Immunogen

Synthetic peptide within n-terminal human noggin.

Host

Rabbit

Positive Control

MG-63, human brain, mouse brain

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

26kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Noggin

SYNONYMS

Nog antibody; NOGG_HUMAN antibody; Noggin antibody; SYM 1 antibody; SYM1 antibody; Symphalangism 1 (proximal) antibody; Synostoses (multiple) syndrome 1 antibody; SYNS 1 antibody; SYNS1 antibody

SEQUENCE SIMILARITIES

Belongs to the noggin family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Noggin is a bone morphogenetic protein (BMP) antagonist expressed in Spemann's organizer. Murine Noggin is expressed in condensing cartilage and immature chondrocytes, as are many BMPs. It is essential for cartilage morphogenesis and joint formation. Defects in NOG are the cause of multiple synostoses syndrome 1 (SYNS1), symphalangism proximal syndrome (SYM1) and tarsal-carpal coalition syndrome (TCC).

CITATIONS

  • Zhao, Guiyun et al.

    Cell surface proteomics analysis indicates a neural lineage bias of rat bone marrow mesenchymal stromal cells. | BioMed Research International [2014]