PRODUCT CODE: ET1704-80

NMDAR2A Recombinant Rabbit Monoclonal Antibody [JA31-20] (ET1704-80)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NMDAR2A on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of NMDAR2A on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skin tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
Western blot analysis of NMDAR2A on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NMDAR2A Recombinant Rabbit Monoclonal Antibody [JA31-20] (ET1704-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue lysate, rat brain tissue, rat skin tissue, human brain tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA31-20

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

165 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NMDAR2A

SYNONYMS

EPND antibody; FESD antibody; GluN2A antibody; Glutamate [NMDA] receptor subunit epsilon-1 antibody; Glutamate receptor antibody; Glutamate receptor ionotropic N methyl D aspartate 2A antibody; GRIN 2A antibody; GRIN2A antibody; hNR2A antibody; LKS antibody; N methyl D aspartate receptor channel, subunit epsilon 1 antibody; N Methyl D Aspartate Receptor Subtype 2A antibody; N methyl D aspartate receptor subunit 2A antibody; N-methyl D-aspartate receptor subtype 2A antibody; NMDA receptor subtype 2A antibody; NMDAR 2A antibody; NMDAR2A antibody; NMDE1_HUMAN antibody; NR2A antibody; OTTHUMP00000160135 antibody; OTTHUMP00000174531 antibody

SEQUENCE SIMILARITIES

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.

SUBCELLULAR LOCATION

Cell membrane, postsynaptic cell membrane, dendritic spine, synapse, Cytoplasmic vesicle membrane.

FUNCTION

NMDAR2A is a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning.