PRODUCT CODE: ER1706-72

NLRP3 Rabbit Polyclonal Antibody (ER1706-72)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of NLRP3 on different lysates using anti-NLRP3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Human lung <br />
  Lane 2: Mouse thymus<br />
 Lane 3: THP-1
  • Western blot analysis of NLRP3 on different lysates using anti-NLRP3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Human lung <br />
  Lane 2: Mouse thymus<br />
 Lane 3: THP-1
  • ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NLRP3 on different lysates using anti-NLRP3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human lung
Lane 2: Mouse thymus
Lane 3: THP-1

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NLRP3 Rabbit Polyclonal Antibody (ER1706-72)

Immunogen

Synthetic peptide within human nlrp3 aa 987-1,036 / 1,036.

Host

Rabbit

Positive Control

Human lung tissue lysate, mouse thymus tissue lysate, THP-1, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NLRP3

SYNONYMS

AGTAVPRL antibody; AII/AVP antibody; Angiotensin/vasopressin receptor AII/AVP like antibody; Angiotensin/vasopressin receptor AII/AVP-like antibody; C1orf7 antibody; Caterpiller protein 1.1 antibody; CIAS 1 antibody; CIAS1 antibody; CLR1.1 antibody; Cold autoinflammatory syndrome 1 antibody; Cold autoinflammatory syndrome 1 protein antibody; Cryopyrin antibody; Familial cold autoinflammatory syndrome antibody; FCAS antibody; FCU antibody; LRR and PYD domains-containing protein 3 antibody; Muckle-Wells syndrome antibody; MWS antibody; NACHT antibody; NACHT LRR and PYD containing protein 3 antibody; NALP 3 antibody; NALP3 antibody; NALP3_HUMAN antibody; NLR family pyrin domain containing 3 antibody; NLRP3 antibody; PYPAF 1 antibody; PYPAF1 antibody; PYRIN containing APAF1 like protein 1 antibody; PYRIN-containing APAF1-like protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NLRP family.

TISSUE SPECIFICITY

Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level). Expression in monocytes is very weak (at protein level). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level). Expressed in lung epithelial cells (at protein level). Expressed in chondrocytes. Expressed at low levels in resting osteoblasts.

POST-TRANSLATIONAL MODIFICATION

The disulfide bond in the pyrin domain might play a role in reactive oxygen species-mediated activation.; Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Ubiquitination does not lead to degradation, but inhibits inflammasome activation (By similarity). Deubiquitination is catalyzed by BRCC3 and associated with NLRP3 activation and inflammasome assembly. This process can be induced by the activation of Toll-like receptors (by LPS), through a non-transcriptional pathway dependent on the mitochondrial production of reactive oxygen species, and by ATP.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Secreted.

FUNCTION

Cryopyrin interacts selectively with apoptosis associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA).

CITATIONS

  • Wan, T., Pan, Q., & Ping, Y.

    Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders. Science advances, 7(11), eabe2888.

  • Lei, B., Liu, J., Yao, Z., ...

    NF-κB-Induced Upregulation of miR-146a-5p Promoted Hippocampal Neuronal Oxidative Stress and Pyroptosis via TIGAR in a Model of Alzheimer's Disease. Frontiers in cellular neuroscience, 15, 653881.