NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] (HA724075)
Catalog# HA724075
Rabbit Monoclonal to NLRP3
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA724075) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate
Lane 3: Jurkat cell lysate (low expression)
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724075) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA724075) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate
Lane 3: Jurkat cell lysate (low expression)
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724075) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.