PRODUCT CODE: ER1706-45

NLRC3 Rabbit Polyclonal Antibody (ER1706-45)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.
  • Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.
  • ICC staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NLRC3 Rabbit Polyclonal Antibody (ER1706-45)

Immunogen

Synthetic peptide within human nlrc3 aa 1-50 / 1,065.

Host

Rabbit

Positive Control

Human thymus tissue lysates, HUVEC, MCF-7, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

NLRC3

SYNONYMS

CARD15-like antibody; CARD15-like protein antibody; Caterpiller 16.2 antibody; Caterpiller protein 16.2 antibody; CLR16.2 antibody; FLJ00348 antibody; NLR family CARD domain containing 3 antibody; Nlrc3 antibody; NLRC3_HUMAN antibody; NOD-like receptor C3 antibody; NOD3 antibody; NOD3 protein antibody; Nucleotide-binding oligomerization domain protein 3 antibody; Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody; Protein NLRC3 antibody

SEQUENCE SIMILARITIES

Belongs to the NLRP family.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton.

FUNCTION

NLRC3, short for NOD-like receptor family CARD domain containing 3, is an intracellular protein that plays a role in the immune system. It was previously known as nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 (NOD3) and CLR16.2. NLRC3 inhibits the activity of T cells. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.