Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
All lanes: Western blot analysis of Niemann Pick C1 with anti-Niemann Pick C1 antibody [JB87-33] (ET7107-57) at 1:1,000 dilution.
Lane 1: Wild-type HEK293 whole cell lysate (20 µg).
Lane 2: Niemann Pick C1 knockout HEK293 whole cell lysate (20 µg).
ET7107-57 was shown to specifically react with Niemann Pick C1 in wild-type HEK293 cells. No band was observed when Niemann Pick C1 knockout sample was tested. Wild-type and Niemann Pick C1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-57, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Niemann Pick C1 protein precursor antibody;Niemann Pick disease, type C1 antibody;Niemann-Pick C1 protein antibody;NPC antibody;NPC1 antibody;NPC1_HUMAN antibody
Belongs to the patched family.
Niemann-Pick disease, type C1 (NPC1) is a disease of a membrane protein that mediates intracellular cholesterol trafficking in mammals. In humans the protein is encoded by the NPC1 gene (chromosome location 18q11). NPC1 was identified as the gene that when mutated, results in Niemann-Pick disease, type C. Niemann-Pick disease, type C is a rare neurovisceral lipid storage disorder resulting from autosomal recessively inherited loss-of-function mutations in either NPC1 or NPC2. This disrupts intracellular lipid transport, leading to the accumulation of lipid products in the late endosomes and lysosomes. Approximately 95% of NPC patients are found to have mutations in the NPC1 gene. NPC1 encodes a putative integral membrane protein containing sequence motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.