PRODUCT CODE: ER1802-59

NGF Rabbit Polyclonal Antibody (ER1802-59)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NGF on recombinant protein lysate using anti-NGF antibody at 1/500 dilution.
  • Western blot analysis of NGF on recombinant protein lysate using anti-NGF antibody at 1/500 dilution.
  • ICC staining NGF in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.
Western blot analysis of NGF on recombinant protein lysate using anti-NGF antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NGF Rabbit Polyclonal Antibody (ER1802-59)

Immunogen

Synthetic peptide within human ngf aa 119-158 / 241.

Host

Rabbit

Positive Control

Recombinant protein, N2A, SHG-44, SH-SY-5Y, rat brain tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

NGF

SYNONYMS

Beta nerve growth factor antibody; Beta NGF antibody; Beta-nerve growth factor antibody; Beta-NGF antibody; HSAN5 antibody; MGC161426 antibody; MGC161428 antibody; Nerve growth factor (beta polypeptide) antibody; Nerve growth factor antibody; Nerve growth factor beta antibody; Nerve growth factor beta polypeptide antibody; Nerve growth factor beta subunit antibody; NGF antibody; NGF_HUMAN antibody; NGFB antibody; NID67 antibody

SEQUENCE SIMILARITIES

Belongs to the NGF-beta family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.