Lane 1: Hela cell lysate
Lane 2: Daudi cell lysate
Lane 3: MCF-7 cell lysate
Recombinant Rabbit monoclonal primary
NFkB p100 Recombinant Rabbit Monoclonal Antibody [JM82-03] (ET1705-45)
Hela, MCF-7, A431, human tonsil tissue, human placenta tissue, human colon cancer tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
CVID10 antibody; DNA binding factor KBF2 antibody; H2TF1 antibody; Lymphocyte translocation chromosome 10 protein antibody; LYT 10 antibody; NF kB2 antibody; NFKB p52/p100 subunit antibody; Nuclear factor Kappa B subunit 2 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody; Oncogene Lyt 10 antibody; p100 antibody; Transcription factor NFKB2 antibody
While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.; Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.; Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.
NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.