PRODUCT CODE: EM1701-67

NFIB NF1B2 Mouse Monoclonal Antibody [1H2] (EM1701-67)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.
  • Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.
  • ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

NFIB NF1B2 Mouse Monoclonal Antibody [1H2] (EM1701-67)

Immunogen

Recombinant protein within human nfib / nf1b2 aa 1-420 / 420.

Host

Mouse

Positive Control

SH-SY-5Y, LO-2, rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1H2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NFIB NF1B2

SYNONYMS

CCAAT Box Binding Transcription Factor antibody; CCAAT-box-binding transcription factor antibody; CTF antibody; HMGIC/NFIB antibody; NF-I/B antibody; NF1-B antibody; NF1B antibody; NF1B2 antibody; NFI-B antibody; NFI-RED antibody; Nfib antibody; NFIB_HUMAN antibody; NFIB2 antibody; NFIB3 antibody; Nuclear factor 1 B-type antibody; Nuclear factor 1/B antibody; Nuclear Factor 1B antibody; Nuclear Factor I B antibody; Nuclear factor I/B antibody; TGGCA Binding Protein antibody; TGGCA-binding protein antibody; TRANSCRIPTION FACTOR NFIB antibody

SEQUENCE SIMILARITIES

Belongs to the CTF/NF-I family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.