Neutrophil Elastase Recombinant Rabbit Monoclonal Antibody [JF098-6] (ET1702-78)
Catalog# ET1702-78
Rabbit Monoclonal to Neutrophil Elastase
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WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
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FC
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Human
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Mouse
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Neutrophil Elastase Recombinant Rabbit Monoclonal Antibody [JF098-6] (ET1702-78)
Immunogen
Recombinant protein within human neutrophil elastase aa 1-140 / 268.
Host
Rabbit
Positive Control
HL-60 cell lysates, A549, Hela, HepG2, human bone marrow tissue, mouse bone tissue, human tonsil tissue, human spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF098-6
RRID
APPLICATION DILUTION
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WB
-
1:500-1:2,000
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IF-Cell
-
1:50
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IF-Tissue
-
1:50
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IHC-P
-
1:50-1:200
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FC
-
1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Neutrophil Elastase
SYNONYMS
Bone marrow serine protease antibody;ELA2 antibody;ELANE antibody;Elastase 2 antibody;Elastase 2 neutrophil antibody;Elastase neutrophil expressed antibody;Elastase-2 antibody;ELNE_HUMAN antibody;GE antibody;Granulocyte derived elastase antibody;HLE antibody;HNE antibody;Human leukocyte elastase antibody;Leukocyte elastase antibody;Medullasin antibody;NE antibody;Neutrophil elastase antibody;PMN E antibody;PMN elastase antibody;Polymorphonuclear elastase antibody;SCN1 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase S1 family. Elastase subfamily.
TISSUE SPECIFICITY
Bone marrow cells. Neutrophil.
SUBCELLULAR LOCATION
Cytoplasmic vesicle, phagosome
FUNCTION
Neutrophil Elastase (NE) is a serine protease that is expressed in bone marrow precursor cells, stored in peripheral blood granulocytes, and implicated in the progression of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome and cystic fibrosis. In neutrophils, Neutrophil Elastase contributes largely to the proteolysis of phagocytosed proteins, the migration of neutrophils and the remodeling of tissues following injury. Neutrophil Elastase, which is also designated medullasin, is secreted into the extracellular matrix, where it is then capable of destroying connective tissue proteins, including elastin, proteoglycans and Type IV Collagens. Neutrophil Elastase also mediates proteolysis by cleaving proteins that are associated with the complement system, such as antithrombin and Fibrinogen. Additionally, Neutrophil Elastase functions as a potent platelet agonist, where it potentiates the aggregation, secretion and mobilization of calcium in response to cathepsin G binding to platelet surface receptors.
