Neuronatin Recombinant Rabbit Monoclonal Antibody [JE35-00] (HA722326)
Catalog# HA722326
Rabbit Monoclonal to Neuronatin
Tested for
-
WB
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Neuronatin on different lysates with Rabbit anti-Neuronatin antibody (HA722326) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SK-Br-3 cell lysate (negative)
Lane 3: E14.0 mouse brain tissue lysate
Lane 4: Mouse embryo tissue lysate
Lane 5: Rat embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722326) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: SK-Br-3 cell lysate (negative)
Lane 3: E14.0 mouse brain tissue lysate
Lane 4: Mouse embryo tissue lysate
Lane 5: Rat embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722326) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.