Neurofilament Antibody Sampler Kit (HAK21090)
Catalog# HAK21090
Reacts with
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Human
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Mouse
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Rat
Western blot analysis of Neurofilament heavy polypeptide on different lysates with Rabbit anti-Neurofilament heavy polypeptide antibody (ET1702-72) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 3: Rat hippocampus tissue lysate (20 µg/Lane)
Predicted band size: 200 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-72) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 3: Rat hippocampus tissue lysate (20 µg/Lane)
Predicted band size: 200 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-72) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.