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Nestin Recombinant Rabbit Monoclonal Antibody [PSH07-89] (HA722919)

Price: Regular price $385.00

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Catalog# HA722919

Rabbit Monoclonal to Nestin

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • IHC-Fr

  • IF-Tissue

  • mIHC

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Nestin Recombinant Rabbit Monoclonal Antibody [PSH07-89] (HA722919)

Immunogen

Recombinant protein within mouse nestin aa 700-1,000.

Host

Rabbit

Positive Control

RD cell lysate, Neuro-2a cell lysate, Mouse brain (P0) tissue lysate, Mouse brain (P7) tissue lysate, Mouse brain tissue lysate, U-87 MG, C2C12, C6, human kidney tissue, mouse kidney tissue, rat kidney tissue, mouse embryonic brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH07-89

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IF-Cell

  • 1:1,000-1:5,000

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500-1:2,000

  • IF-Tissue

  • 1:500

  • mIHC

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 207 kDa

Isotype

IgG

TARGET

UNIPROT #

P48681

PROTEIN NAME

Nestin

SYNONYMS

ESTM 46 antibody;FLJ 21841 antibody;FLJ21841 antibody;Intermediate filament protein antibody;Nbla00170 antibody;nes antibody;NEST_HUMAN antibody;Nestin antibody

SUBCELLULAR LOCATION

Cytoplasm, Intermediate filament.

FUNCTION

Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

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