NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] (HA722320)

UNIPROT #

Q8WX92

PROTEIN NAME

NELF-B

SYNONYMS

A730008L03Rik antibody;AB041607 antibody;AI663983 antibody;Cobra1 antibody;Cofactor of BRCA1 antibody;DKFZp586B0519 antibody;KIAA1182 antibody;Negative elongation factor B antibody;Negative elongation factor protein B antibody;Nelf b antibody;NELF-B antibody;NELFB antibody;RGD1307832 antibody;RP13-122B23.3 antibody;RP23-132N23.4 antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

In molecular biology, the NELF (negative elongation factor) is a four-subunit protein complex (NELF-A, NELF-B, NELF-C/NELF-D, and NELF-E) that negatively impacts transcription by RNA polymerase II (Pol II) by pausing about 20-60 nucleotides downstream from the transcription start site (TSS). NELF is located in the nucleus. NELF binds in a stable complex with DSIF (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB)-sensitivity inducing factor) and RNA polymerase II together, but not with either alone. Due to its role in transcription, NELF is also a key player in the negative function of DSIF. NELF also works with DSIF to inhibit the speed of Pol II during the elongation phase in transcription. In D. melanogaster, the HSP70 gene is affected by NELF and DSIF through the induction of promoter proximal pausing. It is thought that NELF arose to assist DSIF by amplifying its negative effects in order to increase gene expression control. P-TEFb (positive transcription elongation factor b) inhibits the effect of NELF and DSIF on Pol II elongation, via its phosphorylation of serine-2 of the C-terminal domain of Pol II, and the SPT5 subunit of DSIF, causing dissociation of NELF.