NEDD4-2 Recombinant Rabbit Monoclonal Antibody [SN73-03] (ET1611-42)
Catalog# ET1611-42
Rabbit Monoclonal to NEDD4-2
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WB
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IP
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Predicted band size: 112 kDa
Observed band size: 130 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NEDD4-2 Recombinant Rabbit Monoclonal Antibody [SN73-03] (ET1611-42)
Immunogen
Synthetic peptide within human nedd4l aa 926-975 / 975.
Host
Rabbit
Positive Control
Jurkat cell lysate, HT-29 cell lysate, HCT 116 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN73-03
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 112 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NEDD4-2
SYNONYMS
E3 ubiquitin protein ligase NEDD4 like protein antibody;E3 ubiquitin-protein ligase NEDD4-like antibody;KIAA0439 antibody;NED4L_HUMAN antibody;NEDD4 2 antibody;NEDD4 2c antibody;Nedd4-2 antibody;NEDD4-2a antibody;NEDD4.2 antibody;NEDD4b antibody;NEDD4L antibody;NEDD4La antibody;NEDD4Lb antibody;NEDD4Lc antibody;NEDD4Ld antibody;NEDD4Le antibody;NEDD4Lf antibody;NEDD4Lg antibody;NEDD4Lh antibody;NEDL3 antibody;Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody;Neural precursor cell expressed, developmentally down regulated 4 like antibody;RSP5 antibody;Ubiquitin protein ligase Rsp5 antibody
TISSUE SPECIFICITY
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.; Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.
SUBCELLULAR LOCATION
Cytoplasm, Endosome, Golgi apparatus.
FUNCTION
NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGFβ RII, NEDD4-L is thought to inhibit the TGFβ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events.
CITATIONS
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Wu Wensi,et al
circGRHPR inhibits aberrant epithelial-mesenchymal transformation progression of lung epithelial cells associated with idiopathic pulmonary fibrosis
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WB
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Human
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Citation
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