PRODUCT CODE: ET7109-09

Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47] (ET7109-09)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1,000 dilution.
  • Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Ndufs4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47] (ET7109-09)

Immunogen

Recombinant protein within human ndufs4 aa 43-175.

Host

Rabbit

Positive Control

Rat heart tissue lysate, rat kidney tissue, human small intestine tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE40-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Ndufs4

SYNONYMS

AQDQ antibody; CI 18 antibody; CI 18 kDa antibody; CI AQDQ antibody; CI-18 kDa antibody; CI-AQDQ antibody; Complex I 18 kDa antibody; Complex I AQDQ antibody; Complex I-18 kDa antibody; Complex I-AQDQ antibody; mitochondrial antibody; mitochondrial respiratory chain complex I (18 KD subunit) antibody; NADH coenzyme Q reductase antibody; NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody; NADH dehydrogenase antibody; NADH ubiquinone oxidoreductase 18 kDa subunit antibody; NADH-ubiquinone oxidoreductase 18 kDa subunit antibody; NDUFS4 antibody; NDUS4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the complex I NDUFS4 subunit family.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the intermembrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is a complicated, multi-protein, L-shaped complex composed of at least 45 different subunits and located in the mitochondrial inner membrane. NDUFS4 (NADH dehydrogenase (ubiquinone) Fe-S protein 4), also known as AQDQ or CI-18 (Complex I-18kDa protein), belongs to the Complex I NDUFS4 subunit family. NDUFS4 localizes to the matrix side of the inner membrane of the mitochondrion and functions as an accessory subunit of Complex I. Mutations in the gene encoding NDUFS4 can result in Complex I mitochondrial respiratory chain deficiency. Patients with this deficiency may exhibit cardiomyopathy, myopathy, liver failure and neurological disorders.