Applications
-
WB
-
IHC-P
-
FC
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47] (ET7109-09)
Immunogen
Recombinant protein within human ndufs4 aa 43-175.
Host
Rabbit
Positive Control
Rat heart tissue lysate, rat kidney tissue, human small intestine tissue, SH-SY-5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE40-47
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
20 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
Ndufs4
SYNONYMS
AQDQ antibody; CI 18 antibody; CI 18 kDa antibody; CI AQDQ antibody; CI-18 kDa antibody; CI-AQDQ antibody; Complex I 18 kDa antibody; Complex I AQDQ antibody; Complex I-18 kDa antibody; Complex I-AQDQ antibody; mitochondrial antibody; mitochondrial respiratory chain complex I (18 KD subunit) antibody; NADH coenzyme Q reductase antibody; NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody; NADH dehydrogenase antibody; NADH ubiquinone oxidoreductase 18 kDa subunit antibody; NADH-ubiquinone oxidoreductase 18 kDa subunit antibody; NDUFS4 antibody; NDUS4_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the complex I NDUFS4 subunit family.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the intermembrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is a complicated, multi-protein, L-shaped complex composed of at least 45 different subunits and located in the mitochondrial inner membrane. NDUFS4 (NADH dehydrogenase (ubiquinone) Fe-S protein 4), also known as AQDQ or CI-18 (Complex I-18kDa protein), belongs to the Complex I NDUFS4 subunit family. NDUFS4 localizes to the matrix side of the inner membrane of the mitochondrion and functions as an accessory subunit of Complex I. Mutations in the gene encoding NDUFS4 can result in Complex I mitochondrial respiratory chain deficiency. Patients with this deficiency may exhibit cardiomyopathy, myopathy, liver failure and neurological disorders.