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PRODUCT CODE: ER1803-14

NDUFS3 Rabbit Polyclonal Antibody (ER1803-14)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: LOVO<br /> Lane 2: MCF-7
  • Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: LOVO<br /> Lane 2: MCF-7
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with NDUFS3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution.
Positive control:
Lane 1: LOVO
Lane 2: MCF-7

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NDUFS3 Rabbit Polyclonal Antibody (ER1803-14)

Immunogen

Recombinant protein within human ndufs3 aa 7-239 / 264.

Host

Rabbit

Positive Control

LOVO, MCF-7, rat epididymis tissue, human liver cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

30 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O75489

PROTEIN NAME

NDUFS3

SYNONYMS

CI 30 antibody; CI 30KD antibody; CI-30kD antibody; Complex I 30KD antibody; Complex I 30kDa subunit antibody; COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody; Complex I-30kD antibody; mitochondrial antibody; NADH coenzyme Q reductase antibody; NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody; NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody; NADH dehydrogenase ubiquinone 30 kDa subunit antibody; NADH-ubiquinone oxidoreductase 30 kDa subunit antibody; NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody; NDUFS3 antibody; NDUS3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the complex I 30 kDa subunit family.

SUBCELLULAR LOCATION

Mitochondrion inner membrane.

FUNCTION

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.