PRODUCT CODE: ER1802-94

NDRG2 Rabbit Polyclonal Antibody (ER1802-94)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.
  • Western blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.
  • ICC staining NDRG2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NDRG2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NDRG2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with NDRG2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NDRG2 Rabbit Polyclonal Antibody (ER1802-94)

Immunogen

Recombinant protein within human ndrg2 1-150 aa.

Host

Rabbit

Positive Control

Mouse brain tissue, mouse skeletal muscle tissue, HepG2, LOVO, SH-SY-5Y, rat brain tissue, human liver tissue, human prostate tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NDRG2

SYNONYMS

Antidepressant related protein ADRG123 antibody; Cytoplasmic protein Ndr1 antibody; DKFZp781G1938 antibody; FLJ25522 antibody; KIAA1248 antibody; N myc downstream regulated gene 2 antibody; N myc downstream regulator 2 antibody; NDR1 related protein NDR2 antibody; NDRG 2 antibody; NDRG family member 2 antibody; NDRG1 related protein antibody; NDRG2 antibody; NDRG2_HUMAN antibody; Protein NDRG2 antibody; Protein Syld709613 antibody; SYLD antibody; Syld709613 protein antibody

SEQUENCE SIMILARITIES

Belongs to the NDRG family.

TISSUE SPECIFICITY

Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).

DEVELOPMENTAL STAGE

Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The N-myc downstream regulated gene (NDRG) family is comprised of four members, namely NDRG1, NDRG2, NDRG3 and NDRG4, all of which share 57-65% homology. NDRG2 (NDRG family member 2), also known as SYLD, is a 371 amino acid protein that localizes to both the cytoplasm and the perinuclear region in neurons. Expressed at high levels in heart, brain, dendritic cells, salivary gland and skeletal muscle and at lower levels in liver and kidney, NDRG2 is thought to be involved in dendritic and neuronal cell differentiation and outgrowth. Additionally, NDRG2 expression is downregulated in a variety of carcinomas, including liver cancer, pancreatic cancer and meningioma, suggesting a possible role for NDRG2 in tumor suppression. NDRG2 is found in brain lesions of Alzheimer Disease (AD)-affected patients and is thought to be associated with the progression of AD. Five isoforms of NDRG2 exist due to alternative splicing events.