PRODUCT CODE: ET7106-57

NCK1 Recombinant Rabbit Monoclonal Antibody [JU41-80] (ET7106-57)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela    <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Rat kidney tissue<br />
 Lane 4: Mouse testis tissue
  • Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela    <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Rat kidney tissue<br />
 Lane 4: Mouse testis tissue
  • ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH-3T3
Lane 3: Rat kidney tissue
Lane 4: Mouse testis tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NCK1 Recombinant Rabbit Monoclonal Antibody [JU41-80] (ET7106-57)

Immunogen

Synthetic peptide within human nck1 aa 212-302 .

Host

Rabbit

Positive Control

NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU41-80

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43/35 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

NCK1

SYNONYMS

Cytoplasmic protein NCK1 antibody; Melanoma Nck protein antibody; MGC12668 antibody; NCK 1 antibody; NCK adaptor protein 1 antibody; NCK alpha antibody; NCK antibody; NCK tyrosine kinase antibody; Nck-1 antibody; NCK1 antibody; NCK1_HUMAN antibody; NCKalpha antibody; Non catalytic region of tyrosine kinase antibody; SH2/SH3 adaptor protein NCK alpha antibody; SH2/SH3 adaptor protein NCK-alpha antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm. Endoplasmic reticulum. Nucleus.

FUNCTION

The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.