Lane 1: Hela
Lane 2: NIH-3T3
Lane 3: Rat kidney tissue
Lane 4: Mouse testis tissue
Recombinant Rabbit monoclonal primary
NCK1 Recombinant Rabbit Monoclonal Antibody [JU41-80] (ET7106-57)
Synthetic peptide within human nck1 aa 212-302 .
NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Cytoplasmic protein NCK1 antibody; Melanoma Nck protein antibody; MGC12668 antibody; NCK 1 antibody; NCK adaptor protein 1 antibody; NCK alpha antibody; NCK antibody; NCK tyrosine kinase antibody; Nck-1 antibody; NCK1 antibody; NCK1_HUMAN antibody; NCKalpha antibody; Non catalytic region of tyrosine kinase antibody; SH2/SH3 adaptor protein NCK alpha antibody; SH2/SH3 adaptor protein NCK-alpha antibody
Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
Cytoplasm. Endoplasmic reticulum. Nucleus.
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.