PRODUCT CODE: 0805-5

NCAM-L1 Rabbit Polyclonal Antibody (0805-5)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NCAML1 on human fetal brain tissue lysate using anti-NCAML1 antibody at 1/1,000 dilution.
  • Western blot analysis of NCAML1 on human fetal brain tissue lysate using anti-NCAML1 antibody at 1/1,000 dilution.
  • ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHG-44 cells with NCAML1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NCAML1 on human fetal brain tissue lysate using anti-NCAML1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NCAM-L1 Rabbit Polyclonal Antibody (0805-5)

Immunogen

Synthetic peptide within human ncam-l1 aa 1,208-1,257 / 1,257.

Host

Rabbit

Positive Control

Human fetal brain tissue, 293T, Hela, human breast cancer tissue, human kidney tissue, mouse kidney tissue, SHG-44.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

220kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NCAM-L1

SYNONYMS

Antigen identified by monoclonal antibody; R1 antibody; CAML1 antibody; CD171 antibody; CD171 antigen antibody; HSAS antibody; HSAS1 antibody; Hyd antibody; L1 antibody; L1 cell adhesion molecule antibody; L1-NCAM antibody; L1cam antibody; L1CAM_HUMAN antibody; MASA antibody; MIC5 antibody; N CAML1 antibody; N-CAM-L1 antibody; NCAM-L1 antibody; NCAML1 antibody; Nerve-growth factor-inducible large external glycoprotein antibody; Neural cell adhesion molecule L1 antibody; NILE antibody; OTTHUMP00000025992 antibody; S10 antibody; SPG1 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

SUBCELLULAR LOCATION

Cell membrane. Cell projection. Membrane.

FUNCTION

The neural cell adhesion molecule L1 (NCAM-L1) is an immunoglobulin superfamily adhesion molecule that is critical for neural development, involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS). Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. Defects in L1CAM are also the cause of mental retardation, aphasia, shuffling gait, and adducted thumbs syndrome (MASA), a X-linked disorder.