NCAM1 / CD56 Recombinant Rabbit Monoclonal Antibody [JF1021] (ET1702-43)
Catalog# ET1702-43
Rabbit Monoclonal to NCAM1
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
-
IP
-
mIHC
-
Human
-
Zebrafish
Lysates/proteins at 10 µg/Lane.
Predicted band size: 95 kDa
Observed band size: 120/140 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-43) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NCAM1 / CD56 Recombinant Rabbit Monoclonal Antibody [JF1021] (ET1702-43)
Immunogen
Synthetic peptide within c-terminal human ncam1.
Host
Rabbit
Positive Control
SH-SY5Y cell lysates, human brain tissue lysates, A549, SH-SY5Y, human tonsil tissue, zebrafish tissue, human kidney tissue, human small lell lung cancer.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF1021
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
Use at an assay dependent concentration.
-
mIHC
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 95 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NCAM1
SYNONYMS
antigen MSK39 identified by monoclonal antibody;5.1H11 antibody;antigen recognized by monoclonal antibody;5.1H11 antibody;CD56 antibody;cell adhesion molecule, neural, 1 antibody;MSK 39 antibody;MSK39 antibody;N-CAM-1 antibody;NCAM 1 antibody;NCAM antibody;NCAM C antibody;NCAM-1 antibody;NCAM1 antibody;NCAM1_HUMAN antibody;NCAMC antibody;Neural cell adhesion molecule 1 antibody;Neural cell adhesion molecule NCAM antibody;OTTHUMP00000235666 antibody
SUBCELLULAR LOCATION
Cell membrane, Secreted.
FUNCTION
Neural cell adhesion molecules (NCAMs) are a family of closely related cell surface glycoproteins involved in cell to cell interactions during growth and thought to play an important role in embryogenesis and development. The expression of these molecules is widespread in all three germ layers during embryogenesis, but is more restrictive in adult tissues. NCAM expression is observed in a variety of human tumors including neuroblastomas, rhabdo-myosarcomas, Wilms' tumor, Ewing's sarcoma and some primitive myeloid malignancies. Multiple isoforms of NCAM have been reported in both mouse and human brain tissue. In humans, NCAMs arise from differential splicing and use of alternative polyadenylation sites of a single gene mapping to 11q23.
CITATIONS
-
Zhu, J., Song, G., Zhou, X., Han, T. L., Yu, X., Chen, H., Mansell, T., Novakovic, B., Baker, P. N., Cannon, R. D., Saffery, R., Chen, C., & Zhang, H.
CD39/CD73 Dysregulation of Adenosine Metabolism Increases Decidual Natural Killer Cell Cytotoxicity: Implications in Unexplained Recurrent Spontaneous Abortion
-
IHC
-
Human
-
Citation
-
-
Kaiyan Wei,et al
FUT8 Regulates Cerebellar Neurogenesis and Development Through Maintaining the Level of Neural Cell Adhesion Molecule Cntn2
-
WB
-
Mouse
-
Citation
-
