PRODUCT CODE: ER1706-30

NaV1.8 Rabbit Polyclonal Antibody (ER1706-30)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Mouse

  • Rat

Western blot analysis of NaV1.8 on rat spinal cord lysate using anti-NaV1.8 antibody at 1/100 dilution.
  • Western blot analysis of NaV1.8 on rat spinal cord lysate using anti-NaV1.8 antibody at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NaV1.8 antibody. Counter stained with hematoxylin.
Western blot analysis of NaV1.8 on rat spinal cord lysate using anti-NaV1.8 antibody at 1/100 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NaV1.8 Rabbit Polyclonal Antibody (ER1706-30)

Immunogen

Synthetic peptide within rat nav18 aa 1,907-1,956 / 1,956.

Host

Rabbit

Positive Control

Rat spinal cord lysate, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

220 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

NaV1.8

SYNONYMS

hPN3 antibody; mPN3 antibody; Peripheral nerve sodium channel 3 antibody; Pn3 (gene name) antibody; PN3 antibody; SCN10A antibody; SCNAA_HUMAN antibody; Sensory neuron sodium channel antibody; Sns (gene name) antibody; SNS antibody; Sodium channel protein type 10 subunit alpha antibody; Sodium channel protein type X alpha subunit antibody; Sodium channel protein type X subunit alpha antibody; Voltage-gated sodium channel alpha subunit Nav1.8 antibody; Voltage-gated sodium channel subunit alpha Nav1.8 antibody

SEQUENCE SIMILARITIES

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

TISSUE SPECIFICITY

Expressed in dorsal root ganglion and trigeminal ganglion.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by NEDD4L; which promotes its endocytosis.; Phosphorylation at Ser-1452 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.; Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 815) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.