PRODUCT CODE: ER1706-57

NaV1.7 Rabbit Polyclonal Antibody (ER1706-57)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

ICC staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NaV1.7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NaV1.7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
ICC staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NaV1.7 Rabbit Polyclonal Antibody (ER1706-57)

Immunogen

Synthetic peptide within human nav17 aa 1,939-1,988 / 1,988.

Host

Rabbit

Positive Control

SH-SY5Y, A549, Hela, human placenta tissue, rat testis tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

226 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

NaV1.7

SYNONYMS

ETHA antibody; GEFSP7 antibody; hNE Na antibody; hNE-Na antibody; hNENa antibody; NE NA antibody; NENA antibody; Neuroendocrine sodium channel antibody; Peripheral sodium channel 1 antibody; PN1 antibody; Scn9a antibody; SCN9A_HUMAN antibody; Sodium channel protein type 9 subunit alpha antibody; Sodium channel protein type IX subunit alpha antibody; Sodium channel voltage gated type IX alpha antibody; Sodium channel voltage gated type IX alpha polypeptide antibody; Sodium channel voltage gated type IX alpha subunit antibody; Voltage gated sodium channel alpha subunit Nav1.7 antibody; Voltage gated sodium channel subunit alpha Nav1 antibody; Voltage-gated sodium channel subunit alpha Nav1.7 antibody

SEQUENCE SIMILARITIES

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.

TISSUE SPECIFICITY

Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.; Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.

SUBCELLULAR LOCATION

Plasma membrane. In neurite terminals.

FUNCTION

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain.