PRODUCT CODE: R1108-2

NANOG Rabbit Polyclonal Antibody (R1108-2)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis on NCCIT (A) and F9 (B) cell lysates using anti-NANOG rabbit polyclonal antibody.
  • Western blot analysis on NCCIT (A) and F9 (B) cell lysates using anti-NANOG rabbit polyclonal antibody.
Western blot analysis on NCCIT (A) and F9 (B) cell lysates using anti-NANOG rabbit polyclonal antibody.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NANOG Rabbit Polyclonal Antibody (R1108-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

NCCIT, F9

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

TARGET

UNIPROT #

PROTEIN NAME

NANOG

SYNONYMS

Embryonic stem cell specific homeobox protein (Nanog) antibody; ENK antibody; FLJ12581 antibody; hNanog antibody; Homeobox protein NANOG antibody; Homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody; NANOG antibody; Nanog homeobox antibody; NANOG_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the Nanog homeobox family.

TISSUE SPECIFICITY

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

DEVELOPMENTAL STAGE

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

SUBCELLULAR LOCATION

Cell nucleus

FUNCTION

NANOG is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. NANOG is thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. Overexpression of NANOG in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent.