Lane 1: Wild-type SH-SY5Y whole cell lysate (20 µg).
Lane 2/3: NOX4 fragment 1 knockdown SH-SY5Y whole cell lysate (20 µg).
Lane 4/5: NOX4 fragment 2 knockdown SH-SY5Y whole cell lysate (20 µg).
ET1607-4 was shown to specifically react with NOX4 in wild-type SH-SY5Y cells. Weakened bands were observed when NOX4 knockdown samples were tested. Wild-type and NOX4 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-4, 1/1,000) and Loading control antibody(Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Recombinant Rabbit monoclonal primary
NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214] (ET1607-4)
Recombinant protein within human nox4 aa 460-578 / 578.
JAR cell lysate, SH-SY5Y cell lysate, Hela, A431, A549, human lung tissue, human kidney tissue, rat stomach tissue, rat kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Use at an assay dependent concentration.
NADPH oxidase 4/NOX4
Kidney oxidase-1 antibody; Kidney superoxide-producing NADPH oxidase antibody; KOX 1 antibody; KOX antibody; Kox-1 antibody; NADPH antibody; NADPH oxidase 4 antibody; Nox4 antibody; NOX4_HUMAN antibody; Renal NAD(P)H-oxidase antibody; RENOX antibody
Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
Expressed in fetal kidney and fetal liver.
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
Cell membrane, Nucleus, Endoplasmic reticulum membrane.
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.
Li, Kaiqun et al.
High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-κB signaling. | Journal of Cellular Physiology